ELISA/assay

Chicken Interferon beta ELISA Kit

MBS737277

48-Strip-Wells

470 USD

ELISA Kit

Chicken Interferon beta ELISA Kit

Interferon beta

Chicken Interferon b ELISA Kit

interferon beta; Interferon beta; interferon beta; IFN-beta; fibroblast interferon; interferon, beta 1, fibroblast; Fibroblast interferon

IFN-beta

IFN-b

IFNB1; IFNB1; IFB; IFF; IFNB; IFB; IFNB; IFN-beta

IFNB_HUMAN

Chicken

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This betaIFN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken betaIFN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the Assay: betaIFN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-betaIFN antibody and an betaIFN-HRP conjugate. The assay sample and buffer are incubated together with betaIFN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the betaIFN concentration since betaIFN from samples and betaIFN-HRP conjugate compete for the anti-betaIFN antibody binding site. Since the number of sites is limited, as more sites are occupied by betaIFN from the sample, fewer sites are left to bind betaIFN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The betaIFN concentration in each sample is interpolated from this standard curve.

4504603

NP_002167.1

NM_002176.2

P01574

22,294 Da

Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028)

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9p21. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; cell surface receptor linked signal transduction; response to exogenous dsRNA; negative regulation of viral genome replication; B cell differentiation; defense response to bacterium; positive regulation of innate immune response; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response

48-Strip-Wells

470 USD

96-Strip-Wells

675