catalog number :
MBS737181
products type :
ELISA Kit
products full name :
Canine Angiotensin 2 ELISA Kit
products short name :
Angiotensin 2
products gene name :
ANG2
specificity :
This assay has high sensitivity and excellent specificity for detection of Ang 2. No significant cross-reactivity or interference between Ang 2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Ang 2 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This Ang 2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine Ang 2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cardiovascular
products description :
Principle of the assay: Ang 2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Ang 2 antibody and an Ang 2-HRP conjugate. The assay sample and buffer are incubated together with Ang 2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ang 2 concentration since Ang 2 from samples and Ang 2-HRP conjugate compete for the anti-Ang 2 antibody binding site. Since the number of sites is limited, as more sites are occupied by Ang 2 from the sample, fewer sites are left to bind Ang 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ang 2 concentration in each sample is interpolated from this standard curve.