ELISA/assay

Monkey Complement 4 ELISA Kit

MBS737069

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Monkey Complement 4 ELISA Kit

Complement 4

complement 4; complement 4; complement C4; MHC-linked complement C4; complement 4

C4

C4; C4-2

Monkey

This assay has high sensitivity and excellent specificity for detection of C4. No significant cross-reactivity or interference between C4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 ng/mL.

Intended Uses: This C4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey C4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: C4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C4. Standards or samples are then added to the microtiter plate wells and C4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C4 are added to each well to "sandwich" the C4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4 concentration in each sample is interpolated from this standard curve.

116175422

NP_001070701.1

NM_001077233.1

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675