catalog number :
MBS737042
products type :
ELISA Kit
products full name :
Goat Immunoglobulin M ELISA Kit
products short name :
[Immunoglobulin M]
other names :
[IgM, partial]
products gene name :
[IgM]
specificity :
This assay has high sensitivity and excellent specificity for detection of IgM. No significant cross-reactivity or interference between IgM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IgM and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Standard Curve/Testing Data
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ug/mL.
products categories :
Immunology
products description :
Intended Uses: This IgM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat IgM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IgM ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IgM antibody and an IgM-HRP conjugate. The assay sample and buffer are incubated together with IgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgM concentration since IgM from samples and IgM-HRP conjugate compete for the anti-IgM antibody binding site. Since the number of sites is limited, as more sites are occupied by IgM from the sample, fewer sites are left to bind IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgM concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAB30943.1
size4 :
10x96-Strip-Wells