ELISA/assay

Canine Matrix Gla Protein ELISA Kit

MBS736991

96 Strip Wells

675 USD

ELISA Kit

Canine Matrix Gla Protein ELISA Kit

Matrix Gla Protein

MGP, partial; Matrix Gla protein; matrix Gla protein; cell growth-inhibiting gene 36 protein; matrix Gla protein; Cell growth-inhibiting gene 36 protein

MGP

MGP; MGP; NTI; GIG36; MGLAP; MGLAP; GIG36; MGP

MGP_HUMAN

Canine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This MGP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MGP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Signal Transduction

Principle of the Assay: MGP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MGP antibody and an MGP-HRP conjugate. The assay sample and buffer are incubated together with MGP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MGP concentration since MGP from samples and MGP-HRP conjugate compete for the anti-MGP antibody binding site. Since the number of sites is limited, as more sites are occupied by MGP from the sample, fewer sites are left to bind MGP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MGP concentration in each sample is interpolated from this standard curve

47496663

CAG29354.1

P08493

15,325 Da

Endochondral Ossification Pathway 198812!!Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway 169349

The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

96 Strip Wells

675 USD