catalog number :
MBS736769
products type :
ELISA Kit
products full name :
Monkey Troponin I ELISA Kit
products short name :
Troponin I
other names :
troponin I; troponin I
products gene name :
Tn- I
other gene names :
LOC100136571
specificity :
This assay has high sensitivity and excellent specificity for detection of TNI. No significant cross-reactivity or interference between TNI and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TNI and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This TNI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey TNI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Signal Transduction
products description :
Principle of the assay: TNI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TNI antibody and an TNI-HRP conjugate. The assay sample and buffer are incubated together with TNI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNI concentration since TNI from samples and TNI-HRP conjugate compete for the anti-TNI antibody binding site. Since the number of sites is limited, as more sites are occupied by TNI from the sample, fewer sites are left to bind TNI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNI concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001117133.1
ncbi gb acc num :
NM_001123661.1