ELISA/assay

Sheep Prostaglandin E2 ELISA Kit

MBS736744

48-Strip-Wells

470 USD

ELISA Kit

Sheep Prostaglandin E2 ELISA Kit

Prostaglandin E2

PGE2

Sheep

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This PGE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PGE2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PGE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGE2 antibody and an PGE2-HRP conjugate. The assay sample and buffer are incubated together with PGE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGE2 concentration since PGE2 from samples and PGE2-HRP conjugate compete for the anti-PGE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGE2 from the sample, fewer sites are left to bind PGE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGE2 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675