ELISA/assay

Monkey Matrix metalloproteinase 9 ELISA Kit

MBS736695

48-Strip-Wells

470 USD

ELISA Kit

Monkey Matrix metalloproteinase 9 ELISA Kit

Matrix metalloproteinase 9

matrix metalloproteinase-9 preproprotein; Matrix metalloproteinase-9; matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

MMP-9

MMP9; MMP9; GELB; CLG4B; MMP-9; MANDP2; CLG4B; MMP-9; GELB

MMP9_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of MMP-9. No significant cross-reactivity or interference between MMP-9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MMP-9 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Cancer

Intended Uses: This MMP-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey MMP-9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay MMP-9 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MMP-9. Standards or samples are then added to the microtiter plate wells and MMP-9 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MMP-9 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MMP-9 are added to each well to "sandwich" the MMP-9 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MMP-9 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-9 concentration in each sample is interpolated from this standard curve.

74272287

NP_004985.2

NM_004994.2

78,458 Da

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Angiogenesis Pathway (198772); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); CXCR4-mediated Signaling Events Pathway (137910); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; EC 3.4.24.35; Secreted; Protease. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity. Biological Process: positive regulation of keratinocyte migration; extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; ossification; macrophage differentiation; positive regulation of apoptosis; ephrin receptor signaling pathway; proteolysis; leukocyte migration; skeletal development; embryo implantation. Disease: Metaphyseal Anadysplasia 2

48-Strip-Wells

470 USD

96-Strip-Wells

675