ELISA/assay

Canine Macrophage colony-stimulating factor ELISA Kit

MBS736646

48-Strip-Wells

470 USD

ELISA Kit

Canine Macrophage colony-stimulating factor ELISA Kit

Macrophage colony-stimulating factor

macrophage colony-stimulating factor; Macrophage colony-stimulating factor 1; macrophage colony-stimulating factor 1; lanimostim; colony stimulating factor 1 (macrophage); LanimostimCleaved into the following chain:Processed macrophage colony-stimulating factor 1

M-CSF

CSF1; CSF1; MCSF; CSF-1; CSF-1; M-CSF; MCSF

CSF1_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of MCSF. No significant cross-reactivity or interference between MCSF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MCSF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This MCSF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MCSF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay MCSF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCSF antibody and an MCSF-HRP conjugate. The assay sample and buffer are incubated together with MCSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCSF concentration since MCSF from samples and MCSF-HRP conjugate compete for the anti-MCSF antibody binding site. Since the number of sites is limited, as more sites are occupied by MCSF from the sample, fewer sites are left to bind MCSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCSF concentration in each sample is interpolated from this standard curve.

187463

AAA59572.1

29,215 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Integrins In Angiogenesis Pathway (137999); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979)

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cytokine. Chromosomal Location of Human Ortholog: 1p13.3. Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; integral to membrane; plasma membrane; receptor complex. Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding. Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; reproductive developmental process; positive regulation of odontogenesis of dentine-containing teeth; positive regulation of multicellular organism growth; osteoclast differentiation; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; monocyte activation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; hemopoiesis; positive regulation of Ras protein signal transduction; inflammatory response; positive regulation of macrophage differentiation; positive regulation of cell-matrix adhesion; regulation of ossification; cell differentiation; positive regulation of cell migration

48-Strip-Wells

470 USD

96-Strip-Wells

675