ELISA/assay

Bovine Interleukin 5 ELISA Kit

MBS736611

48-Strip-Wells

470 USD

ELISA Kit

Bovine Interleukin 5 ELISA Kit

Interleukin 5

interleukin-5; Interleukin-5; interleukin-5; TRF; IL-5; BCGF-II; B-cell growth factor II; T-cell replacing factor; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); interleukin 5; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF

IL-5

Il5; Il5; Il-5; IL-5; BCGF-II; TRF

IL5_RAT

Bovine

This assay has high sensitivity and excellent specificity for detection of IL-5. No significant cross-reactivity or interference between IL-5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-5 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This IL-5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine IL-5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IL-5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-5 antibody and an IL-5-HRP conjugate. The assay sample and buffer are incubated together with IL-5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-5 concentration since IL-5 from samples and IL-5-HRP conjugate compete for the anti-IL-5 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-5 from the sample, fewer sites are left to bind IL-5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-5 concentration in each sample is interpolated from this standard curve.

54019426

NP_068606.1

NM_021834.1

15,207 Da

Allograft Rejection Pathway (83511); Allograft Rejection Pathway (535); Asthma Pathway (83508); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83509); Autoimmune Thyroid Disease Pathway (533); Cytokine Signaling In Immune System Pathway (1011036); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198521)

cytokine with B-cell growth factor activity [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675