ELISA/assay

Bovine Gastrin ELISA Kit

MBS736603

48-Strip-Wells

470 USD

ELISA Kit

Bovine Gastrin ELISA Kit

Gastrin

gastrin; Gastrin; gastrin; preprogastrin; gastrin; Gastrin component I

GT

GAST; GAST; GAS; GAS; G52; G34; G17; G14; G6

GAST_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of GT. No significant cross-reactivity or interference between GT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GT and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This GT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: GT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GT antibody and an GT-HRP conjugate. The assay sample and buffer are incubated together with GT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GT concentration since GT from samples and GT-HRP conjugate compete for the anti-GT antibody binding site. Since the number of sites is limited, as more sites are occupied by GT from the sample, fewer sites are left to bind GT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GT concentration in each sample is interpolated from this standard curve.

182991

AAA52520.1

11,394 Da

G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17. [provided by RefSeq, Jul 2008]

gastrin: Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. Belongs to the gastrin/cholecystokinin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: extracellular region. Molecular Function: protein binding; hormone activity. Biological Process: G-protein coupled receptor protein signaling pathway; signal transduction

48-Strip-Wells

470 USD

96-Strip-Wells

675