ELISA/assay

Chicken Cross - liked C-telopeptides of Type I collagen ELISA Kit

MBS736444

48-Strip-Wells

470 USD

ELISA Kit

Chicken Cross - liked C-telopeptides of Type I collagen ELISA Kit

Cross - liked C-telopeptides of Type I collagen

CICP

Chicken

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This CTx1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CTx1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY CTx1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTx1 antibody and an CTx1-HRP conjug ate.The assay sample and buffer are incubated together with CTx1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTx1 concentration since CTx1 from samples and CTx1-HRP conjugate compete for the anti-CTx1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTx1 from the sample, fewer sites are left to bind CTx1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTx1 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675