ELISA/assay

Monkey Complement Factor B ELISA Kit

MBS736386

96 Strip Wells

675 USD

ELISA Kit

Monkey Complement Factor B ELISA Kit

Complement Factor B

complement factor B preproprotein; Complement factor B; complement factor B; C3 proactivator; C3/C5 convertase; C3 proaccelerator; properdin factor B; B-factor, properdin; glycine-rich beta glycoprotein; glycine-rich beta-glycoprotein; complement factor B; C3/C5 convertase; Glycine-rich beta glycoprotein; GBG; PBF2; Properdin factor BCleaved into the following 2 chains:Complement factor B Ba fragment; Complement factor B Bb fragment

CFB

CFB; CFB; BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14; BF; BFD; GBG

CFAB_HUMAN

Monkey

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This CFB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CFB. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Immunology

Principle of the Assay: CFB ELISAkit applies the quantitative sandwich enzyme immunoassay teclinique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CFB. Standards or samples are then added to the microtiter plate wells and CFB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CFB present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CFB are added to each well to "sandwich" the CFB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CFB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFB concentration in each sample is interpolated from this standard curve.

67782358

NP_001701.2

NM_001710.5

68,872 Da

Activation Of C3 And C5 Pathway 106412!!Alternative Complement Activation Pathway 106410!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Complement Cascade Pathway 106405!!Immune System Pathway 106386!!Initial Triggering Of Complement Pathway 106406!!Innate Immune System Pathway 106387!!Regulation Of Complement Cascade Pathway 576254!!Staphylococcus Aureus Infection Pathway 172846

This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD