Human alpha Lactalbumin ELISA Kit | MyBioSource MBS736351 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human alpha Lactalbumin ELISA Kit

catalog :

MBS736351

quantity :

48-Strip-Wells-(Competitive)

price :

440 USD

product information

catalog number :

MBS736351

products type :

ELISA Kit

products full name :

Human alpha Lactalbumin ELISA Kit

products short name :

[alpha Lactalbumin]

products name syn :

[Human a Lactalbumin ELISA Kit]

other names :

[alpha-lactalbumin; Alpha-lactalbumin; alpha-lactalbumin; lysozyme-like protein 7; lactose synthase B protein; lactalbumin, alpha-; Lactose synthase B protein; Lysozyme-like protein 7]

products gene name :

[alpha-La]

products gene name syn :

[a-La]

other gene names :

[LALBA; LALBA; LYZL7]

uniprot entry name :

LALBA_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of alphaLA. No significant cross-reactivity or interference between alphaLA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alphaLA and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Detection Range: 2.5-50ng/mL. Sensitivity: 1.0ng/mL

other info2 :

Intended Uses: This alphaLA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human alphaLA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: alphaLA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for alphaLA. Standards or samples are then added to the microtiter plate wells and alphaLA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of alphaLA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for alphaLA are added to each well to "sandwich" the alphaLA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain alphaLA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alphaLA concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4504947

ncbi acc num :

NP_002280.1

ncbi gb acc num :

NM_002289.2

uniprot acc num :

P00709

ncbi mol weight :

16,225 Da

ncbi pathways :

Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292)

ncbi summary :

This gene encodes alpha-lactalbumin, a principal protein of milk. Alpha-lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and beta 1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic component. Together, these proteins enable LS to produce lactose by transfering galactose moieties to glucose. As a monomer, alpha-lactalbumin strongly binds calcium and zinc ions and may possess bactericidal or antitumor activity. A folding variant of alpha-lactalbumin, called HAMLET, likely induces apoptosis in tumor and immature cells. [provided by RefSeq, Jul 2008]

uniprot summary :

LALBA: Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins. Belongs to the glycosyl hydrolase 22 family. Protein type: Secreted, signal peptide; Transferase; Secreted; Apoptosis. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: extracellular space. Molecular Function: lactose synthase activity; calcium ion binding. Biological Process: cell-cell signaling; apoptosis; defense response to bacterium; signal transduction; lactose biosynthetic process

size1 :

48-Strip-Wells-(Competitive)

price1 :

440 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

440

size3 :

96-Strip-Wells-(Competitive)

price3 :

640

size4 :

96-Strip-Wells-(Sandwich)

price4 :

640

size5 :

5x96-Strip-Wells-(Competitive)

price5 :

2895

size6 :

5x96-Strip-Wells-(Sandwich)

price6 :

2895

size7 :

10x96-Strip-Wells-(Competitive)

price7 :

5415

size8 :

10x96-Strip-Wells-(Sandwich)

price8 :

5415