product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Chicken Brain Derived Neurotrophic Factor ELISA Kit
catalog :
MBS736252
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS736252
products type :
ELISA Kit
products full name :
Chicken Brain Derived Neurotrophic Factor ELISA Kit
products short name :
Brain Derived Neurotrophic Factor
other names :
BDNF, partial; BDNF; BDNF
products gene name :
BDNF
other gene names :
BDNF
uniprot entry name :
D0VPU4_ONCMY
reactivity :
Chicken
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This BDNF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken BDNF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Epigenetics and Nuclear Signaling
products description :
Principle of the Assay: BDNF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BDNF antibody and an BDNF-HRP conjugate. The assay sample and buffer are incubated together with BDNF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BDNF concentration since BDNF from samples and BDNF-HRP conjugate compete for the anti-BDNF antibody binding site. Since the number of sites is limited, as more sites are occupied by BDNF from the sample, fewer sites are left to bind BDNF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDNF concentration in each sample is interpolated from this standard curve.
ncbi gi num :
262342369
ncbi acc num :
ACY54685.1
ncbi mol weight :
27,051 Da
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!