ELISA/assay

Porcine Granulocyte Colony Stimulating Factor ELISA Kit

MBS736180

48-Strip-Wells

470 USD

ELISA Kit

Porcine Granulocyte Colony Stimulating Factor ELISA Kit

Granulocyte Colony Stimulating Factor

granulocyte colony-stimulating factor; Granulocyte colony-stimulating factor; granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin; colony stimulating factor 3 (granulocyte); Pluripoietin

GCSF

CSF3; CSF3; GCSF; CSF3OS; C17orf33; C17orf33; GCSF; G-CSF

CSF3_HUMAN

Porcine

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and (issue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This G-CSF ELISA kit is a 1.5 horn- solid-phase ELISA designed for The quantitative determination of Porcine G-CSF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the Assay: G-CSF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-G-CSF antibody and an G-CSF-HRP conjugate. The assay sample and buffer are incubated together with G-CSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the G-CSF concentration since G-CSF from samples and G-CSF-HRP conjugate compete for the anti-G-CSF antibody binding site. Since the number of sites is limited, as more sites are occupied by G-CSF from the sample, fewer sites are left to bind G-CSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The G-CSF concentration in each sample is interpolated from this standard curve.

297660573

ADI49832.1

P09919

22,293 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Malaria Pathway (152665); Malaria Pathway (152657)

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Subunit structure: Monomer. Subcellular location: Secreted. Post-translational modification: O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). Pharmaceutical use: Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood). Sequence similarities: Belongs to the IL-6 superfamily. Caution: Ref.5 misquotes the gene name as "CSF1".

48-Strip-Wells

470 USD

96-Strip-Wells

675