ELISA/assay

Bovine Phospholipid Scramblase 1 ELISA Kit

MBS736144

48-Strip-Wells

470 USD

ELISA Kit

Bovine Phospholipid Scramblase 1 ELISA Kit

Phospholipid Scramblase 1

phospholipid scramblase 1; Phospholipid scramblase 1; phospholipid scramblase 1; PL scramblase 1; erythrocyte phospholipid scramblase; ca(2+)-dependent phospholipid scramblase 1; phospholipid scramblase 1; Ca(2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; MmTRA1b

PLSCR1

PLSCR1; PLSCR1; MMTRA1B; PL scramblase 1

PLS1_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of PS-1. No significant cross-reactivity or interference between PS-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PS-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PS-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PS-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: PS-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PS-1 antibody and an PS-1-HRP conjugate. The assay sample and buffer are incubated together with PS-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PS-1 concentration since PS-1 from samples and PS-1-HRP conjugate compete for the anti-PS-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PS-1 from the sample, fewer sites are left to bind PS-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PS-1 concentration in each sample is interpolated from this standard curve.

10863877

NP_066928.1

NM_021105.2

26,825 Da

EGFR1 Signaling Pathway (198782)

PLSCR1: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Belongs to the phospholipid scramblase family. Protein type: Membrane protein, integral; Calcium-binding. Chromosomal Location of Human Ortholog: 3q23. Cellular Component: Golgi apparatus; extracellular matrix; membrane; integral to plasma membrane; plasma membrane; nucleolus; cytosol; nucleus; lipid raft. Molecular Function: phospholipid scramblase activity; protein binding; enzyme binding; DNA binding; calcium ion binding; SH3 domain binding; CD4 receptor binding; epidermal growth factor receptor binding. Biological Process: platelet activation; negative regulation of viral genome replication; apoptosis; positive regulation of innate immune response; acute-phase response; positive regulation of transcription from RNA polymerase II promoter; phospholipid scrambling; defense response to virus; regulation of mast cell activation; phosphatidylserine biosynthetic process

48-Strip-Wells

470 USD

96-Strip-Wells

675