ELISA/assay

Canine Matrix metalloproteinase 9 ELISA Kit

MBS736101

48-Strip-Wells

470 USD

ELISA Kit

Canine Matrix metalloproteinase 9 ELISA Kit

Matrix metalloproteinase 9

matrix metalloproteinase-9 preproprotein; Matrix metalloproteinase-9; matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

MMP-9

MMP9; MMP9; GELB; CLG4B; MMP-9; MANDP2; CLG4B; MMP-9; GELB

MMP9_HUMAN

Canine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This MMP-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MMP-9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the Assay: MMP-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP-9 antibody and an MMP-9-HRP conjugate. The assay sample and buffer are incubated together with MMP-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-9 concentration since MMP-9 from samples and MMP-9-HRP conjugate compete for the anti-MMP-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-9 from the sample, fewer sites are left to bind MMP-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-9 concentration in each sample is interpolated from this standard curve.

74272287

NP_004985.2

NM_004994.2

78,458 Da

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Angiogenesis Pathway (198772); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); CXCR4-mediated Signaling Events Pathway (137910); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family. Protein type: Secreted, signal peptide; Protease; EC 3.4.24.35; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity. Biological Process: positive regulation of keratinocyte migration; axon guidance; extracellular matrix organization and biogenesis; ossification; macrophage differentiation; positive regulation of apoptosis; proteolysis; collagen catabolic process; extracellular matrix disassembly; ephrin receptor signaling pathway; skeletal development; leukocyte migration; embryo implantation. Disease: Metaphyseal Anadysplasia 2

48-Strip-Wells

470 USD

96-Strip-Wells

675