ELISA/assay

Chicken C terminal Telopeptides of type l collagen ELISA Kit

MBS736043

96 Strip Wells

675 USD

ELISA Kit

Chicken C terminal Telopeptides of type l collagen ELISA Kit

C terminal Telopeptides of type l collagen

C-C chemokine receptor type 6; C-C chemokine receptor type 6; C-C chemokine receptor type 6; LARC receptor; chemokine receptor-like 3; chemokine (C-C) receptor 6; G protein-coupled receptor 29; G-protein coupled receptor 29; seven-transmembrane receptor, lymphocyte, 22; chemokine (C-C motif) receptor 6; Chemokine receptor-like 3; CKR-L3; DRY6; G-protein coupled receptor 29; GPR-CY4; GPRCY4; LARC receptor; CD_antigen: CD196

CTx1

CCR6; CCR6; BN-1; DCR2; DRY6; CCR-6; CD196; CKRL3; GPR29; CKR-L3; CMKBR6; GPRCY4; STRL22; CC-CKR-6; C-C CKR-6; CKRL3; CMKBR6; GPR29; STRL22; C-C CKR-6; CC-CKR-6; CCR-6; CKR-L3; GPRCY4

CCR6_HUMAN

Chicken

This assay has high sensitivity and excellent specificity for detection of CCR6. No significant cross-reactivity or interference between CCR6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCR6 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This CCR6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CCR6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 ng/mL.

Epigenetics and Nuclear Signaling

Principle of the assay: CCR6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCR6 antibody and an CCR6-HRP conjugate. The assay sample and buffer are incubated together with CCR6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCR6 concentration since CCR6 from samples and CCR6-HRP conjugate compete for the anti-CCR6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCR6 from the sample, fewer sites are left to bind CCR6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCR6 concentration in each sample is interpolated from this standard curve.

37188165

NP_113597.2

NM_031409.3

42,494 Da

Beta Defensins Pathway 530759!!Chemokine Receptors Bind Chemokines Pathway 106359!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Defensins Pathway 530757!!G Alpha (i) Signalling Events Pathway 119550!!GPCR Downstream Signaling Pathway 119548

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD