ELISA/assay

Bovine Lipolysaccharide binding protein ELISA Kit

MBS736006

48-Strip-Wells

470 USD

ELISA Kit

Bovine Lipolysaccharide binding protein ELISA Kit

Lipolysaccharide binding protein

lipopolysaccharide-binding protein; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; LPS-binding protein; BPI fold containing family D, member 2; lipopolysaccharide binding protein

LBP

LBP; LBP; BPIFD2; LBP

LBP_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of LBP. No significant cross-reactivity or interference between LBP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LBP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This LBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine LBP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the Assay: LBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LBP antibody and an LBP-HRP conjugate. The assay sample and buffer are incubated together with LBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LBP concentration since LBP from samples and LBP-HRP conjugate compete for the anti-LBP antibody binding site. Since the number of sites is limited, as more sites are occupied by LBP from the sample, fewer sites are left to bind LBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LBP concentration in each sample is interpolated from this standard curve.

31652249

NP_004130.2

NM_004139.4

53,384 Da

IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Innate Immune System Pathway (106387); NF-kappa B Signaling Pathway (634527); Regulation Of Toll-like Receptor Signaling Pathway (920955); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149); Toll Like Receptor 4 (TLR4) Cascade Pathway (106398); Toll-Like Receptors Cascades Pathway (106388); Toll-like Receptor Signaling Pathway (83076)

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q11.23. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: protein binding; lipopolysaccharide binding; receptor binding. Biological Process: positive regulation of tumor necrosis factor biosynthetic process; detection of molecule of bacterial origin; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; positive regulation of tumor necrosis factor production; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production; positive regulation of interleukin-8 production; lipopolysaccharide transport; defense response to Gram-positive bacterium; macrophage activation during immune response; lipopolysaccharide-mediated signaling pathway; acute-phase response; toll-like receptor signaling pathway; innate immune response; cellular defense response; opsonization; toll-like receptor 4 signaling pathway; positive regulation of macrophage activation

48-Strip-Wells

470 USD

96-Strip-Wells

675