ELISA/assay

Porcine Myelin Basic Protein ELISA Kit

MBS735940

48-Strip-Wells

470 USD

ELISA Kit

Porcine Myelin Basic Protein ELISA Kit

Myelin Basic Protein

MBP, partial; Myelin basic protein; Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein; myelin basic protein; Myelin A1 protein; Myelin membrane encephalitogenic protein

MBP

MBP; MBP; MBP

MBP_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of MBP. No significant cross-reactivity or interference between MBP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MBP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This MBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine MBP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: MBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MBP antibody and an MBP-HRP conjugate. The assay sample and buffer are incubated together with MBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MBP concentration since MBP from samples and MBP-HRP conjugate compete for the anti-MBP antibody binding site. Since the number of sites is limited, as more sites are occupied by MBP from the sample, fewer sites are left to bind MBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBP concentration in each sample is interpolated from this standard curve.

49456793

CAG46717.1

P02686

17,343 Da

Glial Cell Differentiation Pathway (698758); MAPK Cascade Pathway (198834); Neural Crest Differentiation Pathway (672460); Spinal Cord Injury Pathway (739007)

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 18q23. Cellular Component: internode region of axon; compact myelin; cell soma; plasma membrane; nucleus. Molecular Function: protein binding; structural constituent of myelin sheath; protease binding. Biological Process: response to mercury ion; myelination; synaptic transmission; central nervous system development; substantia nigra development; response to toxin; immune response; negative regulation of axonogenesis; axon ensheathment; response to progesterone stimulus; aging

48-Strip-Wells

470 USD

96-Strip-Wells

675