ELISA/assay

Porcine C-telopeptide of type I collagen ELISA Kit

MBS735926

96 Strip Wells

675 USD

ELISA Kit

Porcine C-telopeptide of type I collagen ELISA Kit

C-telopeptide of type I collagen

cardiotoxin isoform 1, cytotoxin isoform 1, CTX-1

CTX-1

Porcine

60

This assay has high sensitivity and excellent specificity for detection of CTX-1. No significant cross-reactivity or interference between CTX-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CTX-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This CTX-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CTX-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Principle of the assay: CTX-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTX-1 antibody and an CTX-1-HRP conjugate. The assay sample and buffer are incubated together with CTX-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTX-1 concentration since CTX-1 from samples and CTX-1-HRP conjugate compete for the anti-CTX-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CTX-1 from the sample, fewer sites are left to bind CTX-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTX-1 concentration in each sample is interpolated from this standard curve.

299268

AAB25732.1

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96 Strip Wells

675 USD