catalog number :
MBS735779
products type :
ELISA Kit
products full name :
Bovine Aggrecan ELISA Kit
products short name :
Aggrecan
other names :
aggrecan core protein; Aggrecan core protein; aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein; aggrecan, structural proteoglycan of cartilage; aggrecan; Cartilage-specific proteoglycan core protein; CSPCP
other gene names :
Acan; Acan; Agc; Agc1; Agc; Agc1; CSPCP
uniprot entry name :
PGCA_RAT
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
products categories :
Signal Transduction
products description :
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This AGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine AGC. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY AGC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGC antibody and an AGC-HRP conjugate. The assay sample and buffer are incubated together with AGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGC concentration since AGC from samples and AGC-HRP conjugate compete for the anti-AGC antibody binding site. Since the number of sites is limited, as more sites are occupied by AGC from the sample, fewer sites are left to bind AGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_071526.1
ncbi gb acc num :
NM_022190.1
ncbi mol weight :
225,171 Da
ncbi pathways :
Endochondral Ossification Pathway (219771); Spinal Cord Injury Pathway (739006)
ncbi summary :
an extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006]