ELISA/assay

Canine Albumin ELISA Kit

MBS735654

48-Strip-Wells

470 USD

ELISA Kit

Canine Albumin ELISA Kit

Albumin

ALB, partial; Serum albumin; serum albumin; albumin (32 AA); albumin (AA 34); growth-inhibiting protein 20; cell growth inhibiting protein 42; albumin

ALB

ALB; ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341; GIG20; GIG42; PRO0903; PRO1708; PRO2044; PRO2619; PRO2675; UNQ696/PRO1341

ALBU_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of Albumin. No significant cross-reactivity or interference between Albumin and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Albumin and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 mg/L.

Intended Uses: This Albumin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine Albumin. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: Albumin ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-Albumin antibody and an Albumin-HRP conjugate. The assay sample and buffer are incubated together with Albumin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Albumin concentration since Albumin from samples and Albumin-HRP conjugate compete for the anti-Albumin antibody binding site. Since the number of sites is limited, as more sites are occupied by Albumin from the sample, fewer sites are left to bind Albumin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Albumin concentration in each sample is interpolated from this standard curve.

37222202

AAQ89947.1

47,360 Da

Bile Acid And Bile Salt Metabolism Pathway (106144); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); HDL-mediated Lipid Transport Pathway (106158); Hemostasis Pathway (106028); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Platelet Activation, Signaling And Aggregation Pathway (106034)

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Carrier; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus. Molecular Function: antioxidant activity; toxin binding; protein binding; copper ion binding; enzyme binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding. Biological Process: receptor-mediated endocytosis; platelet activation; sodium-independent organic anion transport; maintenance of mitochondrion localization; bile acid metabolic process; hemolysis by symbiont of host red blood cells; lipoprotein metabolic process; cellular response to starvation; response to organic substance; bile acid and bile salt transport; response to mercury ion; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; transmembrane transport; positive regulation of circadian sleep/wake cycle, non-REM sleep; response to nutrient; negative regulation of apoptosis. Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic

48-Strip-Wells

470 USD

96-Strip-Wells

675