Canine Fatty Acid Binding Protein 2, Intestinal ELISA Kit | MyBioSource MBS735629 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Fatty Acid Binding Protein 2, Intestinal ELISA Kit

catalog :

MBS735629

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS735629

products type :

ELISA Kit

products full name :

Canine Fatty Acid Binding Protein 2, Intestinal ELISA Kit

products short name :

Fatty Acid Binding Protein 2, Intestinal

other names :

fatty acid binding protein 2, intestinal; Fatty acid-binding protein, intestinal; fatty acid-binding protein, intestinal; fatty acid-binding protein 2; intestinal-type fatty acid-binding protein; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; Intestinal-type fatty acid-binding protein; I-FABP

products gene name :

FABP2

other gene names :

FABP2; FABP2; FABPI; I-FABP; FABPI; I-FABP

uniprot entry name :

FABPI_HUMAN

reactivity :

Canine

specificity :

This assay has high sensitivity and excellent specificity for detection of iFABP2. No significant cross-reactivity or interference between iFABP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iFABP2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This iFABP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine iFABP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: iFABP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-iFABP2 antibody and an iFABP2-HRP conjugate. The assay sample and buffer are incubated together with iFABP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iFABP2 concentration since iFABP2 from samples and iFABP2-HRP conjugate compete for the anti-iFABP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by iFABP2 from the sample, fewer sites are left to bind iFABP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iFABP2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

119594072

ncbi acc num :

EAW73666.1

ncbi mol weight :

15,207 Da

ncbi pathways :

Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

ncbi summary :

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008]

uniprot summary :

FABP2: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. Chromosomal Location of Human Ortholog: 4q28-q31. Cellular Component: cytoplasm. Molecular Function: transporter activity; fatty acid binding. Biological Process: transport; digestion

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage