ELISA/assay

Canine Pepsinogen A ELISA Kit

MBS735622

48-Strip-Wells

470 USD

ELISA Kit

Canine Pepsinogen A ELISA Kit

Pepsinogen A

pepsinogen A; Pepsin A-5; pepsin A-5; pepsinogen-5; pepsinogen A5; pepsinogen 5, group I (pepsinogen A); Pepsinogen-5

PGA

PGA5; PGA5

PEPA5_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of PGA. No significant cross-reactivity or interference between PGA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PGA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PGA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PGA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGA antibody and an PGA-HRP conjugate. The assay sample and buffer are incubated together with PGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGA concentration since PGA from samples and PGA-HRP conjugate compete for the anti-PGA antibody binding site. Since the number of sites is limited, as more sites are occupied by PGA from the sample, fewer sites are left to bind PGA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGA concentration in each sample is interpolated from this standard curve.

387013

AAA60061.1

41,993 Da

Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]

PGA5: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Belongs to the peptidase A1 family. Protein type: EC 3.4.23.1; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q13. Molecular Function: aspartic-type endopeptidase activity. Biological Process: digestion; proteolysis

48-Strip-Wells

470 USD

96-Strip-Wells

675