ELISA/assay

Porcine Fibroblast Growth Factor 7 ELISA Kit

MBS735534

48-Strip-Wells

470 USD

ELISA Kit

Porcine Fibroblast Growth Factor 7 ELISA Kit

Fibroblast Growth Factor 7

FGF7, partial; Fibroblast growth factor 7; fibroblast growth factor 7; FGF-7; keratinocyte growth factor; heparin-binding growth factor 7; fibroblast growth factor 7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor

FGF7

FGF7; FGF7; KGF; HBGF-7; KGF; FGF-7; HBGF-7

FGF7_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of FGF-7. No significant cross-reactivity or interference between FGF-7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FGF-7 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This FGF-7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine FGF-7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay FGF-7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FGF-7 antibody and an FGF-7-HRP conjugate. The assay sample and buffer are incubated together with FGF-7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF-7 concentration since FGF-7 from samples and FGF-7-HRP conjugate compete for the anti-FGF-7 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF-7 from the sample, fewer sites are left to bind FGF-7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF-7 concentration in each sample is interpolated from this standard curve.

49456957

CAG46799.1

Q6FGV5

11,520 Da

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); FGFR Ligand Binding And Activation Pathway (106344)

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]

FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family. Protein type: Cytokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 15q21.2. Cellular Component: Golgi apparatus; extracellular region. Molecular Function: heparin binding; protein binding; growth factor activity; fibroblast growth factor receptor binding; chemoattractant activity. Biological Process: positive regulation of keratinocyte migration; epidermal growth factor receptor signaling pathway; epidermis development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; hair follicle morphogenesis; nerve growth factor receptor signaling pathway; mesenchymal cell proliferation; signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; positive regulation of cell division; actin cytoskeleton reorganization; positive regulation of cell proliferation; insulin receptor signaling pathway; response to wounding; innate immune response; positive regulation of epithelial cell proliferation

48-Strip-Wells

470 USD

96-Strip-Wells

675