catalog number :
MBS735466
products type :
ELISA Kit
products full name :
Bovine Pentosidine ELISA Kit
products short name :
Pentosidine
products gene name :
Pentosidine
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
products description :
Intended Uses: This PTD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PTD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: PTD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTD antibody and an PTD-HRP conjugate. The assay sample and buffer are incubated together with PTD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTD concentration since PTD from samples and PTD-HRP conjugate compete for the anti-PTD antibody binding site. Since the number of sites is limited, as more sites are occupied by PTD from the sample, fewer sites are left to bind PTD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTD concentration in each sample is interpolated from this standard curve.