ELISA/assay

Bovine Transforming Growth Factor beta ELISA Kit

MBS735457

48-Strip-Wells

470 USD

ELISA Kit

Bovine Transforming Growth Factor beta ELISA Kit

Transforming Growth Factor beta

Bovine Transforming Growth Factor b ELISA Kit

transforming growth factor beta, partial; Transforming growth factor beta; Transforming growth factor beta

TGFbeta

TGFb

TGFBI

C7FFS5_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of TGF-beta. No significant cross-reactivity or interference between TGF-beta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TGF-beta and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Cancer

Intended Uses: This TGF-beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine TGF-beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay TGF-beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGF-beta antibody and an TGF-beta-HRP conjugate. The assay sample and buffer are incubated together with TGF-beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGF-beta concentration since TGF-beta from samples and TGF-beta-HRP conjugate compete for the anti-TGF-beta antibody binding site. Since the number of sites is limited, as more sites are occupied by TGF-beta from the sample, fewer sites are left to bind TGF-beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGF-beta concentration in each sample is interpolated from this standard curve.

254596903

ACT75673.1

4,760 Da

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]

betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium. Protein type: Secreted, signal peptide; Extracellular matrix; Secreted; Cell adhesion. Chromosomal Location of Human Ortholog: 5q31. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; plasma membrane; extracellular region; basement membrane; trans-Golgi network. Molecular Function: collagen binding; integrin binding; protein binding; extracellular matrix binding. Biological Process: cell proliferation; extracellular matrix organization and biogenesis; visual perception; response to stimulus; chondrocyte differentiation; angiogenesis; negative regulation of cell adhesion; cell adhesion. Disease: Corneal Dystrophy, Epithelial Basement Membrane; Corneal Dystrophy, Lattice Type Iiia; Corneal Dystrophy, Avellino Type; Corneal Dystrophy Of Bowman Layer, Type Ii; Corneal Dystrophy, Lattice Type I; Corneal Dystrophy Of Bowman Layer, Type I; Corneal Dystrophy, Groenouw Type I

48-Strip-Wells

470 USD

96-Strip-Wells

675