ELISA/assay

Canine Thymus Activation Regulated Chemokine ELISA Kit

MBS735420

48-Strip-Wells

470 USD

ELISA Kit

Canine Thymus Activation Regulated Chemokine ELISA Kit

Thymus Activation Regulated Chemokine

thymus and activation-regulated chemokine; CC chemokine ABCD-2; chemokine (C-C motif) ligand 17; chemokine (C-C motif) ligand 17; small inducible cytokine subfamily A17; chemokine (C-C motif) ligand 17; CC chemokine ABCD-2; Chemokine (C-C motif) ligand 17; Thymus and activation-regulated chemokine

TARC

Ccl17; Ccl17; Tarc; Abcd-2; Scya17; Scya17l; ABCD-2; TARC; mCG_12286

Q9WUZ6_MOUSE

Canine

This assay has high sensitivity and excellent specificity for detection of TARC. No significant cross-reactivity or interference between TARC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TARC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This TARC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TARC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay TARC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TARC antibody and an TARC-HRP conjugate. The assay sample and buffer are incubated together with TARC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TARC concentration since TARC from samples and TARC-HRP conjugate compete for the anti-TARC antibody binding site. Since the number of sites is limited, as more sites are occupied by TARC from the sample, fewer sites are left to bind TARC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TARC concentration in each sample is interpolated from this standard curve.

5102778

CAB45256.1

Q9WUZ6

10,467 Da

Chemokine Receptors Bind Chemokines Pathway (1000944); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); GPCR Ligand Binding Pathway (1000941); Peptide Ligand-binding Receptors Pathway (1000943); Signal Transduction Pathway (970540)

48-Strip-Wells

470 USD

96-Strip-Wells

675