Canine Glycogen Phosphorylase lsoenzyme BB ELISA Kit | MyBioSource MBS735391 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Glycogen Phosphorylase lsoenzyme BB ELISA Kit

catalog :

MBS735391

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS735391

products type :

ELISA Kit

products full name :

Canine Glycogen Phosphorylase lsoenzyme BB ELISA Kit

products short name :

Glycogen Phosphorylase lsoenzyme BB

other names :

glycogen phosphorylase, brain form; Glycogen phosphorylase, brain form; glycogen phosphorylase, brain form; glycogen phosphorylase B; phosphorylase, glycogen; brain

products gene name :

GPBB

other gene names :

PYGB; PYGB; GPBB

uniprot entry name :

PYGB_HUMAN

reactivity :

Canine

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This GPBB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GPBB. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intra-assay Precision: This assay has high sensitivity and excellent specificity for detection of GPBB. No significant cross-reactivity or interference between GPBB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPBB and all the analogues, therefore, cross reaction may still exist in some cases.

products description :

Principle of the assay: GPBB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GPBB. Standards or samples are then added to the microtiter plate wells and GPBB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GPBB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GPBB are added to each well to “sandwich” the GPBB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GPBB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPBB concentration in each sample is interpolated from this standard curve.

ncbi gi num :

21361370

ncbi acc num :

NP_002853.2

ncbi gb acc num :

NM_002862.3

ncbi mol weight :

96,696 Da

ncbi pathways :

Disease Pathway (530764); Glucose Metabolism Pathway (106199); Glycogen Metabolism Pathway (198856); Glycogen Breakdown (glycogenolysis) Pathway (106212); Glycogen Storage Diseases Pathway (980468); Insulin Signaling Pathway (83090); Insulin Signaling Pathway (501); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196)

ncbi summary :

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]

uniprot summary :

PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family. Protein type: EC 2.4.1.1; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Phosphorylase; Transferase. Chromosomal Location of Human Ortholog: 20p11.21. Cellular Component: membrane; cytoplasm. Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding. Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage