catalog number :
MBS735320
products type :
ELISA Kit
products full name :
Mouse Tartrate Resistant Acid Phosphatase+B742 ELISA Kit
products short name :
Tartrate Resistant Acid Phosphatase+B742
other names :
traP; Protein TraP
products gene name :
TRAP
other gene names :
traP; ECOK12F079
uniprot entry name :
TRAP_ECOLI
specificity :
This assay has high sensitivity and excellent specificity for detection of TRACP. No significant cross-reactivity or interference between TRACP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TRACP and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This TRACP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TRACP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the assay: TRACP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TRACP antibody and an TRACP-HRP conjugate. The assay sample and buffer are incubated together with TRACP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRACP concentration since TRACP from samples and TRACP-HRP conjugate compete for the anti-TRACP antibody binding site. Since the number of sites is limited, as more sites are occupied by TRACP from the sample, fewer sites are left to bind TRACP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRACP concentration in each sample is interpolated from this standard curve.
ncbi acc num :
BAA97949.1
ncbi mol weight :
21,961 Da