ELISA/assay

Goat Fibrinogen ELISA Kit

MBS735156

48-Strip-Wells

470 USD

ELISA Kit

Goat Fibrinogen ELISA Kit

Fibrinogen

fibrinogen; Fibrinogen gamma-B chain; fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide; fibrinogen gamma chain<

FBG

FGG; FGG; Gamma'

FIBG_BOVIN

Goat

This assay has high sensitivity and excellent specificity for detection of FBG. No significant cross-reactivity or interference between FBG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FBG and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This FBG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat FBG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: FBG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FBG antibody and an FBG-HRP conjugate. The assay sample and buffer are incubated together with FBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBG concentration since FBG from samples and FBG-HRP conjugate compete for the anti-FBG antibody binding site. Since the number of sites is limited, as more sites are occupied by FBG from the sample, fewer sites are left to bind FBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBG concentration in each sample is interpolated from this standard curve.

350

CAA33562.1

P12799

50,244 Da

Common Pathway (1009072); Complement And Coagulation Cascades Pathway (84312); Complement And Coagulation Cascades Pathway (484); Extracellular Matrix Organization Pathway (1008838); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1009069); GRB2:SOS Provides Linkage To MAPK Signaling For Integrins Pathway (1008640); Hemostasis Pathway (1009046); Integrin AlphaIIb Beta3 Signaling Pathway (1008639); Integrin Cell Surface Interactions Pathway (1008854); Platelet Aggregation (Plug Formation) Pathway (1009064)

48-Strip-Wells

470 USD

96-Strip-Wells

675