ELISA/assay

Canine Actin Beta ELISA Kit

MBS735129

48-Strip-Wells

470 USD

ELISA Kit

Canine Actin Beta ELISA Kit

Actin Beta

ACTB protein, partial; Actin, cytoplasmic 1; actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1; actin, beta; Beta-actinCleaved into the following chain:Actin, cytoplasmic 1, N-terminally processed

ACTb

ACTB; ACTB; BRWS1; PS1TP5BP1

ACTB_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of ACTbeta. No significant cross-reactivity or interference between ACTbeta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACTbeta and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ACTbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ACTbeta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the Assay: ACTbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACTbeta antibody and an ACTbeta-HRP conjugate. The assay sample and buffer are incubated together with ACTbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTbeta concentration since ACTbeta from samples and ACTbeta-HRP conjugate compete for the anti-ACTbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTbeta from the sample, fewer sites are left to bind ACTbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTbeta concentration in each sample is interpolated from this standard curve.

117167827

AAI13037.1

41,737 Da

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Chaperonin-mediated Protein Folding Pathway (106242); Chromatin Modifying Enzymes Pathway (941403)

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family. Protein type: Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; focal adhesion; protein complex; postsynaptic density; cytosol; ribonucleoprotein complex; nucleoplasm; cytoskeleton; membrane; axon; nuclear chromatin; cytoplasm; plasma membrane. Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; kinesin binding; Tat protein binding; nitric-oxide synthase binding; protein kinase binding; ATP binding. Biological Process: intercellular junction assembly and maintenance; de novo posttranslational protein folding; retinal homeostasis; axon guidance; establishment and/or maintenance of chromatin architecture; cellular protein metabolic process; substantia nigra development; protein folding; ephrin receptor signaling pathway; ATP-dependent chromatin remodeling; innate immune response; blood coagulation; cell motility; vascular endothelial growth factor receptor signaling pathway. Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1

48-Strip-Wells

470 USD

96-Strip-Wells

675