ELISA/assay

Sheep Mast cell tryptase ELISA Kit

MBS735113

48-Strip-Wells

470 USD

ELISA Kit

Sheep Mast cell tryptase ELISA Kit

Mast cell tryptase

MCT; Monocarboxylate transporter 6; Monocarboxylate transporter 5; MCT 5; Solute carrier family 16 member 5

MCT

SLC16A5; MCT5; MCT6; MCT 6; MCT 5

MOT6_HUMAN

Sheep

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This MCT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep MCT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the Assay: MCT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCT antibody and an MCT-HRP conjugate. The assay sample and buffer are incubated together with MCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCT concentration since MCT from samples and MCT-HRP conjugate compete for the anti-MCT antibody binding site. Since the number of sites is limited, as more sites are occupied by MCT from the sample, fewer sites are left to bind MCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCT concentration in each sample is interpolated from this standard curve.

2463630

AAC52013.1

54,994 Da

SLC16A5: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. Protein type: Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 17q25.1. Cellular Component: membrane; integral to plasma membrane. Molecular Function: monocarboxylic acid transmembrane transporter activity; symporter activity; lactate transmembrane transporter activity; secondary active monocarboxylate transmembrane transporter activity. Biological Process: monocarboxylic acid transport

48-Strip-Wells

470 USD

96-Strip-Wells

675