ELISA/assay

Sheep Intercellular Adhesion Molecule 1 ELISA Kit

MBS735092

48-Strip-Wells

470 USD

ELISA Kit

Sheep Intercellular Adhesion Molecule 1 ELISA Kit

Intercellular Adhesion Molecule 1

intercellular adhesion molecule 1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; CD_antigen: CD54

ICAM-1

ICAM1; ICAM1; BB2; CD54; P3.58; ICAM-1

ICAM1_HUMAN

Sheep

This assay has high sensitivity and excellent specificity for detection of ICAM-1. No significant cross-reactivity or interference between ICAM-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ICAM-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This ICAM-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ICAM-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: ICAM-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ICAM-1. Standards or samples are then added to the microtiter plate wells and ICAM-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ICAM-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ICAM-1 are added to each well to "sandwich" the ICAM-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ICAM-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ICAM-1 concentration in each sample is interpolated from this standard curve.

167466198

NP_000192.2

NM_000201.2

57,825 Da

Adaptive Immune System Pathway (366160); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine Signaling In Immune System Pathway (366171); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Extracellular Matrix Organization Pathway (576262); Glucocorticoid Receptor Regulatory Network Pathway (138014)

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta. Protein type: Membrane protein, integral; Cell adhesion; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: extracellular space; focal adhesion; cell surface; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane. Molecular Function: viral receptor activity; integrin binding; protein binding; transmembrane receptor activity; receptor activity. Biological Process: entry of virus into host cell; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; leukocyte adhesion; cellular response to nutrient levels; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; response to drug; regulation of cell adhesion; virion attachment, binding of host cell surface receptor; regulation of immune response; negative regulation of calcium ion transport; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to copper ion; positive regulation of actin filament polymerization; positive regulation of vasoconstriction; cell adhesion mediated by integrin; adhesion to host; response to ionizing radiation; leukocyte migration. Disease: Malaria, Susceptibility To

48-Strip-Wells

470 USD

96-Strip-Wells

675