ELISA/assay

Canine Cadherin 5 ELISA Kit

MBS734991

48-Strip-Wells

470 USD

ELISA Kit

Canine Cadherin 5 ELISA Kit

Cadherin 5

cadherin-5 preproprotein; Cadherin-5; cadherin-5; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin 5, type 2 (vascular endothelium); 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD_antigen: CD144

CDH5

CDH5; CDH5; 7B4; CD144; VE-cadherin

CADH5_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of CAD5. No significant cross-reactivity or interference between CAD5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAD5 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CAD5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CAD5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: CAD5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAD5 antibody and an CAD5-HRP conjugate. The assay sample and buffer are incubated together with CAD5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAD5 concentration since CAD5 from samples and CAD5-HRP conjugate compete for the anti-CAD5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CAD5 from the sample, fewer sites are left to bind CAD5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAD5 concentration in each sample is interpolated from this standard curve.

166362713

NP_001786.2

NM_001795.3

74,499 Da

Adherens Junctions Interactions Pathway (119533); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); S1P2 Pathway (138059); Signal Transduction Pathway (477114)

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 16q22.1. Cellular Component: adherens junction; tight junction; membrane; integral to membrane; plasma membrane; intercellular junction; cell junction. Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding. Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation

48-Strip-Wells

470 USD

96-Strip-Wells

675