Monkey Semaphorin 3 ELISA Kit | MyBioSource MBS734990 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Semaphorin 3 ELISA Kit

catalog :

MBS734990

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS734990

products type :

ELISA Kit

products full name :

Monkey Semaphorin 3 ELISA Kit

products short name :

Semaphorin 3

other names :

semaphorin-3A; Semaphorin-3A; semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Semaphorin III; Sema III

products gene name :

SEMA3

other gene names :

SEMA3A; SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; SEMAD; Sema III

uniprot entry name :

SEM3A_HUMAN

reactivity :

Monkey

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This SEMA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey SEMA3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the Assay: SEMA-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SEMA-3 antibody and an SEMA-3-HRP conjugate. The assay sample and buffer are incubated together with SEMA-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SEMA-3 concentration since SEMA-3 from samples and SEMA-3-HRP conjugate compete for the anti-SEMA-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by SEMA-3 from the sample, fewer sites are left to bind SEMA-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEMA-3 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

5174673

ncbi acc num :

NP_006071.1

ncbi gb acc num :

NM_006080.2

ncbi mol weight :

88,889 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523); Sema3A PAK Dependent Axon Repulsion Pathway (119524); Semaphorin Interactions Pathway (119519)

ncbi summary :

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

uniprot summary :

SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted. Chromosomal Location of Human Ortholog: 7p12.1. Cellular Component: membrane; axon; dendrite; extracellular region. Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding. Biological Process: trigeminal nerve structural organization; facial nerve structural organization; axon guidance; regulation of heart rate; apoptosis; olfactory bulb development; dendrite morphogenesis; axonal fasciculation; neuron migration; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development. Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage