ELISA/assay

Chicken Alternative macrophage activation-associated CC chemokine 1 ELISA Kit

MBS734774

96 Strip Wells

675 USD

ELISA Kit

Chicken Alternative macrophage activation-associated CC chemokine 1 ELISA Kit

Alternative macrophage activation-associated CC chemokine 1

alternative macrophage activation-associated CC-chemokine; C-C motif chemokine 18; C-C motif chemokine 18; CC chemokine PARC; CC chemokine ligand 18; chemokine (C-C), dendritic; dendritic cell chemokine 1; small inducible cytokine A18; small-inducible cytokine A18; macrophage inflammatory protein 4; pulmonary and activation-regulated chemokine; alternative macrophage activation-associated CC chemokine 1; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); Alternative macrophage activation-associated CC chemokine 1; AMAC-1; CC chemokine PARC; Dendritic cell chemokine 1; DC-CK1; Macrophage inflammatory protein 4; MIP-4; Pulmonary and activation-regulated chemokine; Small-inducible cytokine A18Cleaved into the following 3 chains:CCL18(1-68); CCL18(3-69); CCL18(4-69)

AMAC-1

CCL18; CCL18; CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18; AMAC1; DCCK1; MIP4; PARC; SCYA18; AMAC-1; DC-CK1; MIP-4

CCL18_HUMAN

Chicken

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This AMAC-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken AMAC-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Cardiovascular

Principle of the Assay: AMAC-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AMAC-1. Standards or samples are then added to the microtiter plate wells and AMAC-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AMAC-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for AMAC-1 are added to each well to "sandwich" the AMAC-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AMAC-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMAC-1 concentration in each sample is interpolated from this standard curve.

4836258

AAD30390.1

9,849 Da

Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for naive T cells, CD4+ and CD8+ T cells and nonactivated lymphocytes, but not for monocytes or granulocytes. This chemokine attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes. It may play a role in both humoral and cell-mediated immunity responses. [provided by RefSeq, Sep 2014]

96 Strip Wells

675 USD