Porcine Neuropeptide Y ELISA Kit | MyBioSource MBS734700 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine Neuropeptide Y ELISA Kit

catalog :

MBS734700

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS734700

products type :

ELISA Kit

products full name :

Porcine Neuropeptide Y ELISA Kit

products short name :

Neuropeptide Y

other names :

neuropeptide Y; Pro-neuropeptide Y; pro-neuropeptide Y; prepro-neuropeptide Y; neuropeptide Y; Neuropeptide tyrosine; NPY

products gene name :

NP-Y

other gene names :

NPY; NPY; PYY4; NPY; CPON

uniprot entry name :

NPY_HUMAN

reactivity :

Porcine

specificity :

This assay has high sensitivity and excellent specificity for detection of NPY. No significant cross-reactivity or interference between NPY and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NPY and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This NPY ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine NPY. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the assay: NPY ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NPY antibody and an NPY-HRP conjugate. The assay sample and buffer are incubated together with NPY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NPY concentration since NPY from samples and NPY-HRP conjugate compete for the anti-NPY antibody binding site. Since the number of sites is limited, as more sites are occupied by NPY from the sample, fewer sites are left to bind NPY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NPY concentration in each sample is interpolated from this standard curve.

ncbi gi num :

189274

ncbi acc num :

AAA59944.1

ncbi mol weight :

10,851 Da

ncbi pathways :

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Endothelin Pathway (672453); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358)

ncbi summary :

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. [provided by RefSeq, Nov 2009]

uniprot summary :

NPY: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. Belongs to the NPY family. Protein type: Hormone; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7p15.1. Cellular Component: Golgi apparatus; extracellular space; cell; extracellular region. Molecular Function: G-protein coupled receptor activity; neuropeptide hormone activity; neuropeptide Y receptor binding; calcium channel regulator activity; receptor binding. Biological Process: blood circulation; behavior; adult feeding behavior; G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; cell proliferation; neuropeptide signaling pathway; positive regulation of appetite; calcium ion transport; regulation of blood pressure; digestion; regulation of appetite; feeding behavior; cerebral cortex development; cell motility; neurite development; central nervous system neuron development

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage