ELISA/assay

Bovine P selectin ELISA Kit

MBS734634

48-Strip-Wells

470 USD

ELISA Kit

Bovine P selectin ELISA Kit

P selectin

P-selectin; P-selectin; P-selectin; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; selectin P (granule membrane protein 140kDa, antigen CD62); CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD_antigen: CD62P

P-selectin

SELP; SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM; GMRP; GRMP; GMP-140; LECAM3; PADGEM

LYAM3_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of PSEL. No significant cross-reactivity or interference between PSEL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PSEL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PSEL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PSEL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

. Principle of the assay: PSEL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PSEL antibody and an PSEL-HRP conjugate. The assay sample and buffer are incubated together with PSEL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSEL concentration since PSEL from samples and PSEL-HRP conjugate compete for the anti-PSEL antibody binding site. Since the number of sites is limited, as more sites are occupied by PSEL from the sample, fewer sites are left to bind PSEL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSEL concentration in each sample is interpolated from this standard curve.

157419154

NP_002996.2

NM_003005.3

90,834 Da

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); IL-3 Signaling Pathway (198881); IL4-mediated Signaling Events Pathway (137933); Malaria Pathway (152665); Malaria Pathway (152657); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050)

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family. Protein type: Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q22-q25. Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; integral to plasma membrane; cytoplasm; plasma membrane; platelet dense granule membrane; external side of plasma membrane. Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; glycosphingolipid binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding. Biological Process: platelet activation; positive regulation of leukocyte migration; response to lipopolysaccharide; defense response to Gram-negative bacterium; positive regulation of phosphoinositide 3-kinase cascade; heterophilic cell adhesion; leukocyte adhesion; platelet degranulation; regulation of integrin activation; inflammatory response; cell adhesion; leukocyte tethering or rolling; blood coagulation; leukocyte migration. Disease: Ige Responsiveness, Atopic

48-Strip-Wells

470 USD

96-Strip-Wells

675