ELISA/assay

Chicken Interleukin 4 ELISA Kit

MBS734586

48-Strip-Wells

440 USD

ELISA Kit

Chicken Interleukin 4 ELISA Kit

[Interleukin 4]

[interleukin-4; Interleukin-4; interleukin-4; IL-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1]

[IL4]

[IL4; IL4; IL-4; BSF-1]

IL4_PIG

Chicken

This assay has high sensitivity and excellent specificity for detection of IL-36beta. No significant cross-reactivity or interference between IL-36beta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-36beta and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This IL-36beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IL-36beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IL-36beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-36beta antibody and an IL-36beta-HRP conjugate. The assay sample and buffer are incubated together with IL-36beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-36beta concentration since IL-36beta from samples and IL-36beta-HRP conjugate compete for the anti-IL-36beta antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-36beta from the sample, fewer sites are left to bind IL-36beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-36beta concentration in each sample is interpolated from this standard curve.

55742622

NP_999288.1

NM_214123.1

Q04745

15,023 Da

Allograft Rejection Pathway (84530); Allograft Rejection Pathway (535); Asthma Pathway (84527); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84528); Autoimmune Thyroid Disease Pathway (533); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Fc Epsilon RI Signaling Pathway (84494); Fc Epsilon RI Signaling Pathway (493)

Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-4/IL-13 family.

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415