ELISA/assay

Bovine Insulin-like growth factor-2 ELISA Kit

MBS734487

48-Strip-Wells

470 USD

ELISA Kit

Bovine Insulin-like growth factor-2 ELISA Kit

Insulin-like growth factor-2

insulin-like growth factor II; Insulin-like growth factor II; insulin-like growth factor II; insulin-like growth factor type 2; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor 2; Somatomedin-ACleaved into the following 3 chains:Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; Preptin

IGF-2

IGF2; IGF2; IGF-II; PP9974; C11orf43; PP1446; IGF-II

IGF2_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of IGF-2. No significant cross-reactivity or interference between IGF-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGF-2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This IGF-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine IGF-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: IGF-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGF-2 antibody and an IGF-2-HRP conjugate. The assay sample and buffer are incubated together with IGF-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGF-2 concentration since IGF-2 from samples and IGF-2-HRP conjugate compete for the anti-IGF-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGF-2 from the sample, fewer sites are left to bind IGF-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGF-2 concentration in each sample is interpolated from this standard curve.

189955

AAA60088.1

26,331 Da

Apoptosis Pathway (198797); Cardiac Progenitor Differentiation Pathway (712094); Endochondral Ossification Pathway (198812); Hedgehog Signaling Pathway (198835); Hemostasis Pathway (106028); IGF1R Signaling Cascade Pathway (730346); IRS-related Events Triggered By IGF1R Pathway (730348); Metabolism Of Proteins Pathway (106230); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050)

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; plasma membrane; extracellular region. Molecular Function: insulin-like growth factor receptor binding; protein serine/threonine kinase activator activity; protein binding; growth factor activity; hormone activity; receptor activator activity; insulin receptor binding. Biological Process: response to nicotine; positive regulation of catalytic activity; genetic imprinting; multicellular organismal development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; female pregnancy; positive regulation of activated T cell proliferation; response to estradiol stimulus; response to radiation; platelet degranulation; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; skeletal development; response to nutrient levels; response to drug; platelet activation; positive regulation of mitosis; glucose metabolic process; memory; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; cellular protein metabolic process; insulin receptor signaling pathway; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of insulin receptor signaling pathway; blood coagulation. Disease: Silver-russell Syndrome; Growth Restriction, Severe, With Distinctive Facies; Beckwith-wiedemann Syndrome; Wilms Tumor 1

48-Strip-Wells

470 USD

96-Strip-Wells

675