Chicken Somatostatin ELISA Kit | MyBioSource MBS734341 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Chicken Somatostatin ELISA Kit

catalog :

MBS734341

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS734341

products type :

ELISA Kit

products full name :

Chicken Somatostatin ELISA Kit

products short name :

Somatostatin

other names :

Somatostatin; Somatostatin; somatostatin; somatostatin-14; somatostatin-28; growth hormone release-inhibiting factor; somatostatin; Growth hormone release-inhibiting factor

products gene name :

SST

other gene names :

SST; SST; SMST

uniprot entry name :

SMS_HUMAN

reactivity :

Chicken

specificity :

This assay has high sensitivity and excellent specificity for detection of SST. No significant cross-reactivity or interference between SST and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SST and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

products categories :

Neurobiology

products description :

Intended Uses: This SST ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken SST. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: SST ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SST antibody and an SST-HRP conjugate. The assay sample and buffer are incubated together with SST-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SST concentration since SST from samples and SST-HRP conjugate compete for the anti-SST antibody binding site. Since the number of sites is limited, as more sites are occupied by SST from the sample, fewer sites are left to bind SST-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SST concentration in each sample is interpolated from this standard curve.

ncbi gi num :

21619156

ncbi acc num :

AAH32625.1

uniprot acc num :

P61278

ncbi mol weight :

12,736 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. [provided by RefSeq, Jul 2008]

uniprot summary :

SST: Somatostatin inhibits the release of somatotropin. Belongs to the somatostatin family. Protein type: Secreted; Hormone; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q28. Cellular Component: extracellular space; cell soma; extracellular region. Molecular Function: hormone activity. Biological Process: response to drug; G-protein coupled receptor protein signaling pathway; synaptic transmission; negative regulation of cell proliferation; hyperosmotic response; cell surface receptor linked signal transduction; cell-cell signaling; induction of apoptosis by hormones; response to steroid hormone stimulus; response to heat; digestion; response to amino acid stimulus; regulation of cell migration; response to nutrient

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage