ELISA/assay

Porcine C-Type Natriuretic Peptide ELISA Kit

MBS734321

48-Strip-Wells

470 USD

ELISA Kit

Porcine C-Type Natriuretic Peptide ELISA Kit

C-Type Natriuretic Peptide

C-type natriuretic peptide; C-type natriuretic peptide; C-type natriuretic peptide; natriuretic peptide precursor C; natriuretic peptide C

CNP

NPPC; NPPC; CNP; CNP2; CNP2

ANFC_HUMAN

Porcine

This assay has high sensitivity and excellent specificity for detection of CNP. No significant cross-reactivity or interference between CNP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This CNP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CNP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: CNP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNP antibody and an CNP-HRP conjugate. The assay sample and buffer are incubated together with CNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNP concentration since CNP from samples and CNP-HRP conjugate compete for the anti-CNP antibody binding site. Since the number of sites is limited, as more sites are occupied by CNP from the sample, fewer sites are left to bind CNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNP concentration in each sample is interpolated from this standard curve.

13249346

NP_077720.1

NM_024409.2

13,246 Da

The protein encoded by this gene is proteolytically processed to form a secreted hormone of the natriuretic peptide family. The encoded hormone regulates the growth and differentiation of cartilaginous growth plate chondrocytes and may also be vasoactive and natriuretic. [provided by RefSeq, Sep 2011]

NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family. Protein type: Secreted; Hormone; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q37.1. Cellular Component: extracellular space; secretory granule. Molecular Function: protein homodimerization activity; peptide hormone receptor binding; hormone activity; receptor binding. Biological Process: response to drug; cGMP biosynthetic process; ossification; regulation of smooth muscle cell proliferation; regulation of cAMP metabolic process; regulation of multicellular organism growth; negative regulation of meiotic cell cycle; negative regulation of DNA metabolic process; positive regulation of vasodilation; post-embryonic development; positive regulation of osteoblast differentiation; negative regulation of cell proliferation; response to ethanol; positive regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; response to hypoxia; regulation of cGMP metabolic process; regulation of blood vessel size

48-Strip-Wells

470 USD

96-Strip-Wells

675