ELISA/assay

Porcine A Disintegrin And Metalloprotease ELISA Kit

MBS734287

48-Strip-Wells

470 USD

ELISA Kit

Porcine A Disintegrin And Metalloprotease ELISA Kit

A Disintegrin And Metalloprotease

A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin and metalloproteinase with thrombospondin motifs 7; COMPase; ADAMTS-7; ADAM-TS 7; metalloprotease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 7; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7; COMPase

ADAM

Adamts7; Adamts7; ADAM-TS7; ADAMTS7B; ADAM-TS 7; ADAM-TS7; ADAMTS-7

ATS7_MOUSE

Porcine

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Cardiovascular

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This ADAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ADAM. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY ADAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM antibody and an ADAM-HRP conjugate. The assay sample and buffer are incubated together with ADAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM concentration since ADAM from samples and ADAM-HRP conjugate compete for the anti-ADAM antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM from the sample, fewer sites are left to bind ADAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM concentration in each sample is interpolated from this standard curve.

133778949

NP_001003911.2

NM_001003911.2

177,712 Da

Metabolism Of Proteins Pathway (970583); O-glycosylation Of TSR Domain-containing Proteins Pathway (1000404); O-linked Glycosylation Pathway (1000403); Post-translational Protein Modification Pathway (1000373)

ADAMTS7: Metalloprotease that may play a role in the degradation of COMP. Protein type: Motility/polarity/chemotaxis; EC 3.4.24.-; Secreted, signal peptide; Protease; Secreted; Cell surface. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; cell surface; extracellular region. Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding. Biological Process: proteolysis involved in cellular protein catabolic process; negative regulation of chondrocyte differentiation; proteolysis

48-Strip-Wells

470 USD

96-Strip-Wells

675