ELISA/assay

Monkey 17-Alpha Hydroxylase ELISA Kit

MBS734275

96 Strip Wells

675 USD

ELISA Kit

Monkey 17-Alpha Hydroxylase ELISA Kit

17-Alpha Hydroxylase

steroid 17-alpha-hydroxylase/17,20 lyase; Steroid 17-alpha-hydroxylase/17,20 lyase; steroid 17-alpha-hydroxylase/17,20 lyase; CYPXVII; cytochrome P450c17; cytochrome P450-C17; cytochrome P450 17A1; cytochrome p450 XVIIA1; steroid 17-alpha-monooxygenase; 17-alpha-hydroxyprogesterone aldolase; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; cytochrome P450, family 17, subfamily A, polypeptide 1; 17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase

S17AH

CYP17A1; CYP17A1; CPT7; CYP17; S17AH; P450C17; CYP17; S17AH; Cytochrome P450c17

CP17A_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of S17AH. No significant cross-reactivity or interference between S17AH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S17AH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This S17AH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey S17AH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Principle of the assay: S17AH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S17AH antibody and an S17AH-HRP conjugate. The assay sample and buffer are incubated together with S17AH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S17AH concentration since S17AH from samples and S17AH-HRP conjugate compete for the anti-S17AH antibody binding site. Since the number of sites is limited, as more sites are occupied by S17AH from the sample, fewer sites are left to bind S17AH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S17AH concentration in each sample is interpolated from this standard curve.

4503195

NP_000093.1

NM_000102.3

57,371 Da

Androgen Biosynthesis Pathway 106154!!Biological Oxidations Pathway 105698!!C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway 413383!!C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway 468268!!C21-Steroid Hormone Biosynthesis, Progesterone = Cortisol/cortisone Pathway 413395!!C21-Steroid Hormone Biosynthesis, Progesterone = Cortisol/cortisone Pathway 468370!!Cytochrome P450 - Arranged By Substrate Type Pathway 105700!!Endogenous Sterols Pathway 105701!!Glucocorticoid Mineralcorticoid Metabolism Pathway 198902!!Glucocorticoid Biosynthesis Pathway 106152

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD