ELISA/assay

Canine Interferon alpha ELISA Kit

MBS734186

48-Strip-Wells

470 USD

ELISA Kit

Canine Interferon alpha ELISA Kit

Interferon alpha

Canine Interferon a ELISA Kit

interferon-alpha; Interferon alpha-8; interferon alpha-8; leIF B; IFN-alpha-8; interferon alpha-B; interferon alpha-B'; interferon alpha-B2; interferon alpha type 201; interferon, alpha 8; Interferon alpha-B; LeIF B; Interferon alpha-B2

IFN-alpha

IFN-a

IFNA8; IFNA8; IFN-alphaB; IFN-alpha-8; LeIF B

IFNA8_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of IFNalpha. No significant cross-reactivity or interference between IFNalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFNalpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This IFNalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IFNalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the assay: IFNalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IFNalpha. Standards or samples are then added to the microtiter plate wells and IFNalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IFNalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IFNalpha are added to each well to "sandwich" the IFNalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFNalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNalpha concentration in each sample is interpolated from this standard curve.

306902

AAA52716.1

21,989 Da

Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Hepatitis B Pathway (694606)

IFNA8: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family. Protein type: Secreted, signal peptide; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space; extracellular region. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein

48-Strip-Wells

470 USD

96-Strip-Wells

675