ELISA/assay

Canine UL16 Binding protein 2 ELISA Kit

MBS734130

48-Strip-Wells

470 USD

ELISA Kit

Canine UL16 Binding protein 2 ELISA Kit

UL16 Binding protein 2

UL16 binding protein 2; NKG2D ligand 2; NKG2D ligand 2; N2DL-2; NKG2DL2; ALCAN-alpha; UL16-binding protein 2; retinoic acid early transcript 1H; retinoic acid early transcript 1 H; UL16 binding protein 2; ALCAN-alpha; Retinoic acid early transcript 1H; UL16-binding protein 2

ULBP2

ULBP2; ULBP2; N2DL2; RAET1H; N2DL2; RAET1H; UNQ463/PRO791; N2DL-2; NKG2DL2

N2DL2_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of ULBP2. No significant cross-reactivity or interference between ULBP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ULBP2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ULBP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ULBP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: ULBP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ULBP2 antibody and an ULBP2-HRP conjugate. The assay sample and buffer are incubated together with ULBP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ULBP2 concentration since ULBP2 from samples and ULBP2-HRP conjugate compete for the anti-ULBP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ULBP2 from the sample, fewer sites are left to bind ULBP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ULBP2 concentration in each sample is interpolated from this standard curve.

21961213

AAH34689.1

27,368 Da

Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490)

This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6. [provided by RefSeq, Jul 2015]

ULBP2: Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family. Protein type: Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 6q25. Cellular Component: extracellular space; anchored to plasma membrane; cell surface. Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding. Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity; natural killer cell activation

48-Strip-Wells

470 USD

96-Strip-Wells

675