ELISA/assay

Sheep L-selectin ELISA Kit

MBS734115

48-Strip-Wells

470 USD

ELISA Kit

Sheep L-selectin ELISA Kit

L-selectin

L-selectin; L-selectin; L-selectin; gp90-MEL; pln homing receptor; lymph node homing receptor; lymphocyte adhesion molecule 1; leukocyte surface antigen Leu-8; CD62 antigen-like family member L; leukocyte-endothelial cell adhesion molecule 1; selectin L; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL; CD_antigen: CD62L

L-selectin

SELL; SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1; LNHR; LYAM1; LAM-1; LECAM1

LYAM1_HUMAN

Sheep

This assay has high sensitivity and excellent specificity for detection of LS. No significant cross-reactivity or interference between LS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LS and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This LS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep LS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: LS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LS antibody and an LS-HRP conjugate. The assay sample and buffer are incubated together with LS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LS concentration since LS from samples and LS-HRP conjugate compete for the anti-LS antibody binding site. Since the number of sites is limited, as more sites are occupied by LS from the sample, fewer sites are left to bind LS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LS concentration in each sample is interpolated from this standard curve.

5852072

CAB55488.1

Q9UJ43

43,618 Da

Adaptive Immune System Pathway (366160); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413)

This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q23-q25. Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane. Molecular Function: heparin binding; protein binding; protease binding; cell adhesion molecule binding; glycosphingolipid binding; carbohydrate binding. Biological Process: regulation of immune response; cell adhesion; blood coagulation; leukocyte migration; response to ATP. Disease: Iga Nephropathy, Susceptibility To, 1

48-Strip-Wells

470 USD

96-Strip-Wells

675